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Registro Completo |
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
08/06/2018 |
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Data da última atualização: |
29/04/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BOSCO, D. D.; SINSKI, I.; RITSCHEL, P. S.; CAMARGO, U. A.; FAJARDO, T. V. M.; HARAKAVA, R.; QUECINI, V. |
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Afiliação: |
DANIELA DAL BOSCO, CNPUV; IRACI SINSKI, CNPUV; PATRICIA SILVA RITSCHEL, CNPUV; Umberto A. Camargo; THOR VINICIUS MARTINS FAJARDO, CNPUV; Ricardo Harakava; VERA MARIA QUECINI, CNPUV. |
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Título: |
Expression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Transgenic Research, p. 1-18, 2018. [Online] |
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DOI: |
https://doi.org/10.1007/s11248-018-0082-1 |
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Idioma: |
Português |
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Conteúdo: |
Increased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis MenosIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and... Mostrar Tudo |
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Palavras-Chave: |
Fungus; Pathogenesis related protein 5. |
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Thesaurus Nal: |
Chitinase; Epigenetics; Grapevine leafroll-associated virus 3; Vitis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178409/1/s11248-018-0082-1-Artigo-v-publicada-Metilacao-DNA-Transgenic-Research-06-06-18.pdf
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Marc: |
LEADER 02824naa a2200277 a 4500
001 2092380
005 2019-04-29
008 2018 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-018-0082-1$2DOI
100 1 $aBOSCO, D. D.
245 $aExpression of disease resistance in genetically modified grapevines correlates with the contents of viral sequences in the T-DNA and global genome methylation.$h[electronic resource]
260 $c2018
520 $aIncreased tolerance to pathogens is an important goal in conventional and biotechnology-assisted grapevine breeding programs worldwide. Fungal and viral pathogens cause direct losses in berry production, but also affect the quality of the final products. Precision breeding strategies allow the introduction of resistance characters in elite cultivars, although the factors determining the plant?s overall performance are not fully characterized. Grapevine plants expressing defense proteins, from fungal or plant origins, or of the coat protein gene of grapevine leafroll-associated virus 3 (GLRaV-3) were generated by Agrobacterium -mediated transformation of somatic embryos and shoot apical meristems. The responses of the transformed lines to pathogen challenges were investigated by biochemical, phytopathological and molecular methods. The expression of a Metarhizium anisopliae chitinase gene delayed pathogenesis and disease progression against the necrotrophic pathogen Botrytis cinerea. Modified lines expressing a Solanum nigrum osmotin-like protein also exhibited slower disease progression, but to a smaller extent. Grapevine lines carrying two hairpin- inducing constructs had lower GLRaV-3 titers when challenged by grafting, although disease symptoms and viral multiplication were detected. The levels of global genome methylation were determined for the genetically engineered lines, and correlation analyses demonstrated the association between higher levels of methylated DNA and larger portions of virus-derived sequences. Resistance expression was also negatively correlated with the contents of introduced viral sequences and genome methylation, indicating that the effectiveness of resistance strategies employing sequences of viral origin is subject to epigenetic regulation in grapevine. Keywords Chitinase, Epigenetics, Fungus, Grapevine leafroll-associated virus 3, Pathogenesis related protein 5, Vitis
650 $aChitinase
650 $aEpigenetics
650 $aGrapevine leafroll-associated virus 3
650 $aVitis
653 $aFungus
653 $aPathogenesis related protein 5
700 1 $aSINSKI, I.
700 1 $aRITSCHEL, P. S.
700 1 $aCAMARGO, U. A.
700 1 $aFAJARDO, T. V. M.
700 1 $aHARAKAVA, R.
700 1 $aQUECINI, V.
773 $tTransgenic Research, p. 1-18, 2018. [Online]
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Registro original: |
Embrapa Uva e Vinho (CNPUV) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Trigo. Para informações adicionais entre em contato com cnpt.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
05/12/1994 |
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Data da última atualização: |
26/09/2013 |
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Autoria: |
VASCONCELLOS, N. J. S. de; PANDOLFI, V.; PRESTES, A. M.; BARBOSA, M. M.; MORAES FERNANDES, M. I. B. de. |
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Título: |
Recuperação da fertilidade em híbridos resultantes dos cruzamentos entre Triticum aestivum e Agropyron elongatum. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
In: REUNIÃO NACIONAL DE PESQUISA DE TRIGO, 17., 1994, Passo Fundo. Resumos... Passo Fundo: EMBRAPA-CNPT, 1994. |
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Páginas: |
p. 150 |
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Idioma: |
Português |
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Palavras-Chave: |
Espécie afim. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00636naa a2200181 a 4500
001 1824711
005 2013-09-26
008 1994 bl uuuu u00u1 u #d
100 1 $aVASCONCELLOS, N. J. S. de
245 $aRecuperação da fertilidade em híbridos resultantes dos cruzamentos entre Triticum aestivum e Agropyron elongatum.
260 $c1994
300 $ap. 150
653 $aEspécie afim
700 1 $aPANDOLFI, V.
700 1 $aPRESTES, A. M.
700 1 $aBARBOSA, M. M.
700 1 $aMORAES FERNANDES, M. I. B. de
773 $tIn: REUNIÃO NACIONAL DE PESQUISA DE TRIGO, 17., 1994, Passo Fundo. Resumos... Passo Fundo: EMBRAPA-CNPT, 1994.
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Embrapa Trigo (CNPT) |
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